When was rubisco discovered

J Appl Crystallogr — Isme J — Nature —4. Article Google Scholar. Biochemistry-Us — Plant J — Plant Physiol — Methods Enzymol — J Exp Bot — Limnol Oceanogr — Nucleic Acids Res W Soini J, Ukkonen K, Neubauer P High cell density media for Escherichia coli are generally designed for aerobic cultivations - consequences for large-scale bioprocesses and shake flask cultures.

Microb Cell Fact Int J Syst Evol Micr — Metab Eng — Environ Microbiol — Whitney SM, Andrews TJ Photosynthesis and growth of tobacco with a substituted bacterial rubisco mirror the properties of the introduced enzyme.

Febs Lett — AMB Express Int J Mol Sci. Bioresour Technol — Download references. You can also search for this author in PubMed Google Scholar.

ZC and YL conceived and designed the project. JZ performed enzyme screening, characterization and structure analysis. GL designed the carbon capture model and performed associated analysis. AIC constructed the phylogenetic tree.

RHW performed soluble expression analysis and analyzed data. All authors read and approved the final manuscript. Correspondence to Robert H. Wilson , Zhen Cai or Yin Li. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material.

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Zhang, J. Discovery of a readily heterologously expressed Rubisco from the deep sea with potential for CO 2 capture. Download citation. Received : 02 June Accepted : 27 August Published : 07 September Anyone you share the following link with will be able to read this content:.

Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Skip to main content. Search all SpringerOpen articles Search. Download PDF. Carvajal 3 , Robert H. Introduction Photosynthesis uses light energy to convert inorganic CO 2 into organic carbohydrates and forms the basis of most life on earth. Full size image. Materials and method Plasmids construction Seven Rubisco enzymes-encoding genes were selected and synthesized according to their NCBI accession numbers Additional file 1 : Additional Rubisco sequences information.

Rubisco expression and purification A single colony of the E. Rubisco activity assay Carboxylation activity of Rubisco in cellular solution protein was assayed using NaH 13 CO 3 Sigma as substrate in an anaerobic chamber. Metabolic flux analysis The constrained metabolic flux was analyzed according to the Rubisco-based CO 2 capture model.

Kinetic parameters and solubility of RPE Rubisco Both RPE and Rubiscos were purified by nickel affinity chromatography after fusion with a his 6 -tag at the C terminus of the large subunit. Availability of data and materials The data and the materials are all available in this article as well as the Additional files 1 and 2. Wilson Authors Junli Zhang View author publications. View author publications. Ethics declarations Ethics approval and consent to participate Not applicable.

Consent for publication Not applicable. Competing interest The authors declare no competing interest with regard to this manuscript. Additional information Publisher's Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Supplementary Information. Photosynthetica — Google Scholar. Jordan DB and Chollet R Inhibition of ribulose bisphosphate carboxylase by substrate ribulose 1,5-bisphosphate.

J Biol Chem — Annu Rev Plant Physiol — Laing WA and Christeller JT A model for the kinetics of activation and catalysis of ribulose 1,5-bisphosphate carboxylase.

Biochem J — Lendzian KJ Activation of ribulose-1,5-bisphosphate carboxylase by chloroplast metabolites in a reconstituted spinach chloroplast system. Planta — Lorimer GH Evidence for the existence of discrete activator and substrate sites for CO 2 on ribulose-1,5-bisphosphate carboxylase. Lorimer GH, Badger MR and Andrews TJ The activation of ribulose-1,5-bisphosphate carboxylase by carbon dioxide and magnesium ions: equilibria, kinetics, a suggested mechanism, and physiological implications.

Biochemistry — Machler F and Nosberger J Regulation of ribulose bisphosphate carboxylase activity in intact wheat leaves by light, CO 2 , and temperature. J Exp Bot — Perchorowicz JT and Jensen RG Photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlings. The effect of preliminary incubation of substrates, metal ion and enzyme on activity.

Biochem Zeit 7— Assumptions about the evolutionary process that led to the formation of RubisCO adapted from ref. Not all of these proteins are carboxylases. Phylogenetic analysis of the genes encoding these proteins suggests a three-step evolutionary pathway Figure 3 [8]. Cover image. Isolation, enzymatic properties and auxin content of spinach cytoplasmic proteins.

High molecular weight protein comprising a large part of the cytoplasmic proteins. The carboxylation and oxygenation of ribulose 1,5-bisphosphate: The primary events in photosynthesis and photorespiration. Plant Physiol. Plant Physiol Biochem. Original written by Greg Watry. Note: Content may be edited for style and length. Science News. Journal Reference : Douglas M. Banda, Jose H. Pereira, Albert K.

Liu, Douglas J. Adams, Jillian F. Banfield, Patrick M. Novel bacterial clade reveals origin of form I Rubisco. Nature Plants , ; DOI: ScienceDaily, 31 August